BHLHB5 Antibody (Center)

• Catalog Number: orb1930822
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB25879
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Mouse
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 36997 Da
• Target: This BHLHB5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 236-264 amino acids from the Central region of human BHLHB5.
• UniProt ID: Q8NFJ8
• NCBI: NP_689627.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Class E basic helix-loop-helix protein 22, bHLHe22
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

BHLHB5 Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

BHLHB5 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the BHLHB5 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Western blot analysis of BHLHB5 Antibody (Center) in HL-60 cell line lysates (35 ug/lane). BHLHB5 (arrow) was detected using the purified Pab.