Beta galactosidase

• Catalog Number: orb348746
• Category: Tools
• Description: Biotin Beta Galactosidase Conjugated
• Tested applications: DOT, ELISA, IHC, WB
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:8,000 - 1:32,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Form/Appearance: Liquid (sterile filtered)
• Purity: Biotin Beta Galactosidase Conjugated was prepared from electrophoretically pure Beta Galactosidase and Biotin. Biotin Beta Galactosidase assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Beta Galactosidase (E.coli) and anti-Biotin.
• Conjugation: Biotin
• Hazard Information: Non-Toxic
• Storage: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing Beta Galactosidase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of opening product.
• Buffer/Preservatives: 0.05 M Potassium Phosphate, 0.001M Magnesium Chloride, 0.1M Beta-Mercaptoethanol; pH 7.8
• Alternative names: Beta D galactosidase , Beta gal , Beta galactosida
• Note: For research use only
• Application notes: Biotin Beta Galactosidase Conjugated has been tested by ELISA and dot blot and is assayed against 1.0 ug of Streptavidin in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:3,000 is suggested for Biotin Beta Galactosidase Conjugated.
• Expiration Date: 12 months from date of receipt.