beta Actin (phospho-Tyr53) antibody

• Catalog Number: orb506128
• Category: Antibodies
• Description: beta Actin (phospho-Tyr53) antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Bovine, Gallus, Porcine, Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human beta Actin around the phosphorylation site of Tyr53 (DS(p-Y)VG)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, Flow-Cyt=1ug/Test, IHC-F=1:100-500, ICC=1:100, IF=1:100-500, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 42 kDa
• Target: phospho-beta Actin (Tyr53)
• UniProt ID: P60709
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: beta Actin (phospho Y53); p-beta Actin (phospho Y5
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry of NIH/3T3.Primary Antibody (green line): Rabbit Anti-phospho-beta Actin (Tyr53) antibody; Dilution:1μg /10^6 cells.

Western blot:Lane 1:Hela (Human) Cell;Lane 2:MCF-7 (Human) Cell;Lane 3:Jurkat (Human) Cell;Primary:Anti-phospho-beta Actin (Tyr53) at 1:1000 dilution.

Western blot:Pancreas (Mouse);Primary:Anti-phospho-beta Actin (Tyr53) at 1:1000 dilution.