ATG4D antibody

• Catalog Number: orb20182
• Category: Antibodies
• Description: Goat polyclonal antibody to ATG4D
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC
• Reactivity: Canine, Human, Mouse, Porcine, Rat
• Dilution range: ELISA: 1:32000, WB: 1 μg/ml, IHC-P: 2.5 μg/ml
• Conjugation: Unconjugated
• MW: 52.9
• Target: ATG4D
• Entrez: 84971
• Protein Sequence: CSQLAQPTLRLPRT
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti ATG4D antibody, anti ATG4 autophagy related 4
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave an approx. 26kDa band in Human Skeletal Muscle lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 52.9kDa according to NP_116274.3. The 26kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Expiration Date: 12 months from date of receipt.