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ASPA Antibody (N-term)

Catalog Number: orb1929755

DispatchUsually dispatched within 5-10 working days
$ 140.00
Catalog Numberorb1929755
CategoryAntibodies
DescriptionPurified Rabbit Polyclonal Antibody (Pab)
Species/HostRabbit
ClonalityPolyclonal
Clone NumberRB20918
Tested applicationsFC, IF, IHC-P, WB
ReactivityHuman
IsotypeRabbit IgG
Antibody TypePrimary Antibody
Dilution rangeIF: 1:10~50, WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50
Form/AppearancePurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
ConjugationUnconjugated
MW35735 Da
TargetThis ASPA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 82-110 amino acids from the N-terminal region of human ASPA.
UniProt IDP45381
NCBINP_001121557.1, NP_000040.1
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Alternative namesAspartoacylase, Aminoacylase-2, ACY-2, ASPA, ACY2,
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NoteFor research use only
Expiration Date12 months from date of receipt.
ASPA Antibody (N-term)

Flow cytometric analysis of MDA-MB468 cells using ASPA Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram) FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

ASPA Antibody (N-term)

Confocal immunofluorescent analysis of ASPA Antibody (N-term) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

ASPA Antibody (N-term)

Formalin-fixed and paraffin-embedded human skeletal muscle reacted with ASPA Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

ASPA Antibody (N-term)

Western blot analysis of ASPA Antibody (N-term) in MCF7 cell line lysates (35 ug/lane). ASPA (arrow) was detected using the purified Pab.

ASPA Antibody (N-term)

Western blot analysis of ASPA (arrow) using rabbit polyclonal ASPA Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ASPA gene.