apolipoprotein D Antibody

• Catalog Number: orb1248280
• Category: Antibodies
• Description: apolipoprotein D Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: EIA, ELISA, IHC, WB
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: EIEKIPTTFENGRC
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 25kDa band observed in Human Liver and Kidney lysates (calculated MW of 21.3kDa according to NP_001638.1). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:Paraffin embedded Human Kidney and Adrenal Gland. Recommended concentration: 5ug/ml.Enzyme immunoassay:This antibody is reported to work in sandwich-type ELISA both as capturer and as reporter antibody in combination with EB12282.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Approx 25kDa
• Target: apolipoprotein D
• UniProt ID: P05090
• NCBI: NP_001638.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: APOD, apolipoprotein D, apo-D
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 25kDa band observed in Human Liver and Kidney lysates (calculated MW of 21.3kDa according to NP_001638.1). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:Paraffin embedded Human Kidney and Adrenal Gland. Recommended concentration: 5ug/ml.Enzyme immunoassay:This antibody is reported to work in sandwich-type ELISA both as capturer and as reporter antibody in combination with EB12282.
• Expiration Date: 12 months from date of receipt.

orb1248280 (0.3 ug/ml) staining of Human Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1248280 (5 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1248280 (5 ug/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.