Anti-Integrin alpha 7 Antibody

• Catalog Number: orb1473611
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Integrin alpha 7
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IH, WB
• Reactivity: Human
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Integrin alpha 7. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB (1/500 - 1/1000), IH (1/50 - 1/100), IF/IC (1/50 - 1/100), FC (1/20 - 1/50)
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: ITGA7
• Entrez: 3679
• UniProt ID: Q13683
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Integrin alpha-7
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Integrin alpha 7 expression in Hela (A), A431 (B), Jurkat (C) whole cell lysates. (Predicted band size: 128 kD; Observed band size: 129 kD)

Immunohistochemical analysis of Integrin alpha 7 staining in human skeletal muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of Integrin alpha 7 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Overlay histogram showing U2OS cells stained with Anti-Integrin alpha 7 Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by Anti-PGK1 Antibody for 60 min at 37 °C. The secondary antibody used was Goat Anti-Rabbit IgG (H&L) - AF488 at 1/200 dilution for 40 min at 37 °C. Isotype control antibody (blue line) was rabbit igG (1μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.