Anti-HECW2 Antibody

• Catalog Number: orb1422978
• Category: Antibodies
• Description: Rabbit polyclonal antibody to HECW2.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: HECW2
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Dilution range: WB: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), IF: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
• Conjugation: Unconjugated
• UniProt ID: Q9P2P5
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: KIAA1301; NEDL2; E3 ubiquitin-protein ligase HECW2
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescent analysis of HECW2 staining in A549 Cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Western blot analysis of HECW2 expression in H1792 (A), A549 (B), PMVEC (C), AML12 (D) whole cell lysates.