Anti-FOXO3 (pS253) Antibody

• Catalog Number: orb1422419
• Category: Antibodies
• Description: Rabbit polyclonal antibody to FOXO3 (pS253).
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: FOXO3
• Tested applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Dilution range: WB: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500), IP (1/10 - 1/100), IF: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500), IP (1/10 - 1/100)
• Conjugation: Unconjugated
• UniProt ID: O43524
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: FKHRL1; FOXO3A; Forkhead box protein O3; AF6q21 pr
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescent analysis of FOXO3 (pS253) staining in HeLa Cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Immunohistochemical analysis of FOXO3 (pS253) staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Western blot analysis of FOXO3 (pS253) expression in HCT116 (A), Raw264.7 (B) whole cell lysates.