Anti-Collagen 4 alpha 3 Antibody

• Catalog Number: orb1421891
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Collagen 4 alpha 3.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: COL4A3
• Tested applications: IF, IHC, WB
• Reactivity: Human
• Dilution range: WB: WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/100 - 1/500), IF: WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/100 - 1/500)
• Conjugation: Unconjugated
• UniProt ID: Q01955
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Collagen alpha-3(IV) chain; Goodpasture antigen
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Collagen 4 alpha 3 expression in HEK293T (A), SGC7901 (B) whole cell lysates.

Immunohistochemical analysis of Collagen 4 alpha 3 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of Collagen 4 alpha 3 staining in COS7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.