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Item 1 of 2
Anti-CDK1/2/3 (pT14) Antibody
Catalog Number: orb736898
| Catalog Number | orb736898 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to CDK1 |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | CDK1; CDK2; CDK3 |
| Tested applications | IF, WB |
| Reactivity | Human, Mouse, Rat |
| Dilution range | WB: 1/500 - 1/1000, IF: 1/50 - 1/200 |
| Conjugation | Unconjugated |
| UniProt ID | P06493 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Anti-CDK1 antibody, anti-CDC2 antibody, anti-CDC28 Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of CDK1/2/3 (pT14) expression in Hela (A), mouse testis (B), rat testis (C) whole cell lysates.
Immunofluorescent analysis of CDK1/2/3 (pT14) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
