Anti-CD40 [1C10]

• Catalog Number: orb1821609
• Category: Antibodies
• Description: Anti-CD40 [1C10]
• Species/Host: Rat
• Clonality: Monoclonal
• Clone Number: 1C10
• Tested applications: ELISA, FC, Functional Studies, IF, IP
• Reactivity: Mouse
• Isotype: Rat IgG1
• Immunogen: The original antibody was generated by immunizing a rat with 20 µg of soluble extracellular domain of recombinant murine CD40 (sCD40).
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• Target: CD40
• UniProt ID: P27512
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: TNFRSF5; B-cell surface antigen CD40; Bp50; CD40;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using the Anti-CD40 antibody 1C10. Paraformaldehyde fixed mouse splenocytes were stained with anti-unknown specificity antibody (orb256449; isotype control, black line) or the mouse IgG1 version of 1C10 (orb1821609, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of mouse splenocytes cells with Anti-CD40 1C10. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes cells immobilized on Shi-fix™ plus cover-slips stained with the chimeric mouse IgG1 version of 1C10 (orb1821609) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane staining in subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1821609, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256449) followed by staining with Alexa Fluor® 488 secondary antibody.

Western Blot using Anti-CD40 antibody 1C10. Mouse spleen(A) (2 µg/ml), thymus(B) (2 µg/ml), mouse lymph node(C) (1 ug/ml) and lung(D) (1 ug/ml) tissue lysates (35 µg protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric mouse version of 1C10 (orb1821609) before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.