Anti-CD244 [OX-122]

• Catalog Number: orb2645712
• Category: Antibodies
• Description: Anti-CD244 [OX-122]
• Species/Host: Rat
• Clonality: Monoclonal
• Clone Number: OX-122
• Tested applications: Blocking, FC, WB
• Reactivity: Mouse
• Isotype: IgG2a
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• Target: CD244
• UniProt ID: Q07763
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: 2B4; Natural killer cell receptor 2B4; NK cell typ
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of fixed mouse splenocytes with anti-CD244 antibody OX-122. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of OX-122 (orb2645713) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb2645713, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-unknown specificity antibody followed by Alexa Fluor® 488 secondary antibody.

Western Blot using anti-CD244 antibody OX-122 Mouse thymus lysate (A) and mouse spleen lysate (B) samples (35 µg protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit IgG version of OX-122 (orb2645713) at 2 and 1 µg/ml, respectively, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for CD244 is 44.8kDa.