CD124 (pY497) Antibody

SKU: orb2649828

Description

Rabbit polyclonal antibody to CD124 (pY497)

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution range: WB: WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/100 - 1/500), IF: WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/100 - 1/500)
• Reactivity: Human, Mouse, Porcine, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Clone No.: IL4R

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

Alternative Names

IL4RA; Interleukin-4 receptor subunit alpha; IL-4 receptor subunit alpha; IL-4R subunit alpha; IL-4R-alpha; IL-4RA; CD124

Western blot analysis of CD124 (pY497) expression in Myla2059 (A), SGC7901 (B), mouse testis (C), rat testis (D) whole cell lysates.

Immunohistochemical analysis of CD124 (pY497) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of CD124 (pY497) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Quick Database Links

UniProt

P24394