Anti-BCMA [CA8]

• Catalog Number: orb1821591
• Category: Antibodies
• Description: Anti-BCMA [CA8]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CA8
• Tested applications: ELISA, FC
• Reactivity: Human, Primate
• Isotype: Mouse IgG
• Immunogen: The original mouse parental antibody 118G03 was generated by immunizing SJL mice with recombinant human BCMA/TNFRSF17-Fc chimera (R&D 193-Fc) by using the RIMMS method (Rapid immunization multiple sites). A chimeric version of this antibody was generated and it is called CA8.
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• Target: BCMA
• UniProt ID: Q02224
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CD269; BCM; TNFRSF17; Tumor necrosis factor recept
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using anti-BCMA antibody CA8. Daudi cells were stained with the anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of CA8 (orb1821591, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and the cells were analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of Daudi cells with anti-BCMA antibody CA8. Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of CA8 (orb1821591) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, orb1821591, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.