Anti-ATP8 Antibody

• Catalog Number: orb215488
• Category: Antibodies
• Description: Rabbit polyclonal antibody to MT-ATP8 also known as A6L whose expression can be detected in the brain and heart but is highly expressive in the atrium auricular region and aorta. It is found in the mitochondrion. Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extra membranous catalytic core and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ATP8. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB: 1-500-1-1000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: MT-ATP8
• Entrez: 4509, 17706
• UniProt ID: P03928, P11608, P03930
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti-ATP8 antibody, anti-ATPASE8 antibody, anti-MT
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of ATP8 expression in HepG2 (A), mouse brain (B), rat stomach (C) whole cell lysates. (Predicted band size: 7 kD; Observed band size: 10 kD)