Annexin A1 Antibody

• Catalog Number: orb2635466
• Category: Antibodies
• Description: The ANXA1 gene belongs to the annexin family, and contains 4 annexin repeats. A pair of annexin repeats may form one binding site for calcium and a phospholipid. ANXA1 promotes membrane fusion and is involved in exocytosis. The gene for ANXA1 is upregulated in hairy cell leukemia (HCL), and its protein expression is specific for HCL. Detection of ANXA1 provides a simple, highly sensitive and specific assay for diagnosing HCL. Annexin A1 has also been found to be protective against DNA damage induced by heat in breast cancer cells, suggesting it is involved in tumor suppressive and protective activities, and also is associated with treatment resistance.
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Recombinant full-length human Annexin A1 protein was used as the immunogen for the Annexin A1 antibody.
• UniProt ID: P04083
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Application notes: Optimal dilution of the Annexin A1 antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: ANXA1/6452R
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This Annexin A1 antibody is available for research use only.
• Note: For research use only

IHC staining of FFPE human tonsil tissue with Annexin A1 antibody (clone ANXA1/6452R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human tonsil tissue with Annexin A1 antibody (clone ANXA1/6452R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human A549 cell lysate using Annexin A1 antibody (clone ANXA1/6452R). Predicted molecular weight ~36 kDa.

SDS-PAGE analysis of purified, BSA-free Annexin A1 antibody (clone ANXA1/6452R) as confirmation of integrity and purity.