amyloid beta Antibody [6E10], Rabbit IgG

• Catalog Number: orb1671902
• Category: Antibodies
• Description: amyloid beta Antibody [6E10], Rabbit IgG
• Clonality: Recombinant
• Clone Number: 6E10
• Tested applications: ELISA, EM, IHC, IP, WB
• Reactivity: Human
• Isotype: IgG kappa
• Immunogen: Amyloid-beta peptide.
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: FOXL2
• UniProt ID: P05067
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: amyloid beta-peptide, A-beta
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of human Alzheimer's disease hippocampus tissue using anti-Amyloid Beta antibody, 6E10 Anti-Amyloid Beta staining of paraffin embedded human hippocampus affected by Alzheimer's disease using the rmeric version of 6E10 (orb1671902). Antigen retreival was acheived by microwaving in citrate buffer (pH6)- followed by blocking with protein block serum-free buffer. Primary antibody incubation with orb1671902 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-r HRP secondary antibody for 20 mins followed by Ddiaminobenzidine - and counter-staining with haemotoxylin. Staining of amyloid plaques in the parahippocampal gyrus may be observed. Recommended concentration- 2-4 ug/ml.

Western Blot using anti-Amyloid beta antibody 6E10. Human brain cerebral cortex (A)- frontal cortex (B) and cerebellum (C) lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rsion of 6E10 (orb1671902) at 0.3 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for Amyloid beta is ~35kDa- though this protein is known to form SDS-resistant low-molecular weight oligomers as seen in this blot. orb1671902 successfully detected aggregate human Amyloid beta in human brain cerebral cortex- frontal cortex and cerebellum lysate samples.