AMIGO-1 Antibody: Biotin

• Catalog Number: orb150473
• Category: Antibodies
• Description: Mouse monoclonal antibody against AMIGO-1 conjugated to Biotin
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: L86/33 (Formerly sold as S86-36)
• Tested applications: ELISA, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG1
• Immunogen: Fusion protein amino acids 395-493 (cytoplasmic C-terminus) of mouse AMIGO-4
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000)
• Conjugation: Biotin
• MW: 60-80kDa
• Target: AMIGO1
• Entrez: 229715
• UniProt ID: Q80ZD8
• NCBI: NP_001004293.1
• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS
• Alternative names: AMIGO 1 antibody, AMIGO1 antibody, Adhesion molecu
• Note: For research use only
• Application notes: 1 µg/ml of SMC-438 was sufficient for detection of AMIGO-1 in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-AMIGO-1 Monoclonal Antibody, Clone L86/36. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-AMIGO-1 Monoclonal Antibody at 1:200 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) AMIGO-1 Antibody (D) Composite.