alpha tubulin antibody (FITC)

• Catalog Number: orb44531
• Category: Antibodies
• Description: Mouse Monoclonal to alpha tubulin.
• Clonality: Monoclonal
• Clone Number: TU-01
• Tested applications: FC, ICC
• Reactivity: Aves, Human, Invertebrate, Mouse, Paramecium, Plant, Porcine, Yeast
• Isotype: Mouse IgG1
• Immunogen: Fraction of tubulin purified from porcine brain by two cycles of polymerization - depolymerization.
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 1-4 μg/ml. Intracellular staining.
• Purity: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: FITC
• Target: alpha-Tubulin
• Entrez: 7277
• UniProt ID: Q71U36
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-alpha-tubulin antibody
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 1-4 μg/ml. Intracellular staining.
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry staining of alpha tubulin in HeLa cells using mouse monoclonal antibody TU-01 FITC.

Separation of HeLa cells stained using anti-human alpha-tubulin (TU-01) FITC antibody (concentration in sample 5 μg/ml, red-filled) from HeLa cells stained using mouse IgG1 isotype control (MOPC-21) FITC antibody (concentration in sample 5 μg/ml, same as alpha-tubulin FITC concentration, black-dashed) in flow cytometry analysis (intracellular staining) of HeLa cell suspension.