Alpha Smooth Muscle Actin antibody

• Catalog Number: orb704355
• Category: Antibodies
• Description: Alpha Smooth Muscle Actin antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: 2B10
• Tested applications: FC, ICC, IHC-P, WB
• Predicted Reactivity: Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Actin alpha
• Concentration: 1mg/ml
• Dilution range: WB=1:1000-2000, IHC-P=1:50-200, ICC=1:50, Flow-Cyt=1:50, IHC-F=1:50-200, IF=1:50-200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 42 kDa
• Target: alpha smooth muscle Actin
• UniProt ID: P62736
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: alpha sarcomeric Actin; alpha smooth muscle Actin;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

ICC analysis of HepG2 cell using alpha smooth muscle Actin monoclonal Antibody at 1:50 dilution;DAPI (blue) was used to stain the cell nuclei.

IHC-P analysis of human liver tissue using alpha smooth muscle Actin Monoclonal Antibody at 1:50 dilution.

Western blot:Lane 1:A431 Cell;Lane 2:Hela Cell;Lane 3:NIH/3T3 Cell;Lane 4:mouse heart;Lane 5:mouse smooth muscle;Primary:Anti-alpha smooth muscle Actin at 1:500 dilution.