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Catalog Number | orb21307 |
---|---|
Category | Antibodies |
Description | Rat polyclonal antibody to alpha 2 Macroglobulin |
Species/Host | Rat |
Clonality | Polyclonal |
Form/Appearance | Delipidated, heat inactivated, lyophilized, stable whole antiserum. No preservative added Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal rat serum. No foreign proteins added. |
Conjugation | Unconjugated |
Hazard Information | This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information. |
Source | Alpha-2 macroglobulin ids the major protein in the alpha-2 region of mouse serum after separation by electrophoresis. It is a large polymer and a strong antigen. It forms about one-third in the total of the alpha-2 globulins. The protein is isolated by ammonium sulphate precipitation, ion exchange chromatography, gel filtration and preparative starch block electrophoresis. In the purification of alpha-2 macroglobulin the activation of kallikrein is avoided, since it forms an irreversible bond with aplpha-2 macroglobulin, thus contaminating the final immunogen. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Storage | Storage: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day |
Buffer/Preservatives | Delipidated, heat inactivated, lyophilized, stable whole antiserum. No preservative added Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal rat serum. No foreign proteins added. |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
ELISA, IF, IHC | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC-P, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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