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Catalog Number | orb345054 |
---|---|
Category | Antibodies |
Description | Alanine Transaminase antibody (Biotin) |
Species/Host | Sheep |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Porcine |
Isotype | IgG |
Immunogen | Alanine Transaminase (ALT) [Pig Heart] |
Concentration | 1.0 mg/mL |
Dilution range | ELISA: 1:5,000 - 1:25,000, WB: 1:500 - 1:3,000 |
Form/Appearance | Lyophilized |
Purity | Alanine Transaminase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Sheep Serum as well as purified and partially purified Alanine Transaminase (ALT) [Pig Heart]. Cross reactivity against Alanine Transaminase (ALT) from other sources is unknown. |
Conjugation | Biotin |
UniProt ID | P13191 |
NCBI | 113565 |
Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | sheep anti-Alanine Transaminase Antibody, biotin c Read more... |
Note | For research use only |
Application notes | Anti-Alanine Transaminase has been assayed against 1.0 ug of Alanine Transaminase (ALT) in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product. |
Expiration Date | 12 months from date of receipt. |
ELISA | |
Human | |
Rabbit | |
Polyclonal | |
Biotin |
Goat | |
1.57-100 U/L | |
0.63 U/L |
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