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Alanine Transaminase antibody (Biotin)

Alanine Transaminase antibody (Biotin)

Catalog Number: orb345054

DispatchUsually dispatched within 5-10 working days
$ 753.00
Catalog Numberorb345054
CategoryAntibodies
DescriptionAlanine Transaminase antibody (Biotin)
Species/HostSheep
ClonalityPolyclonal
Tested applicationsELISA, WB
ReactivityPorcine
IsotypeIgG
ImmunogenAlanine Transaminase (ALT) [Pig Heart]
Concentration1.0 mg/mL
Dilution rangeELISA: 1:5,000 - 1:25,000, WB: 1:500 - 1:3,000
Form/AppearanceLyophilized
PurityAlanine Transaminase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Sheep Serum as well as purified and partially purified Alanine Transaminase (ALT) [Pig Heart]. Cross reactivity against Alanine Transaminase (ALT) from other sources is unknown.
ConjugationBiotin
UniProt IDP13191
NCBI113565
StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Buffer/Preservatives0.01% (w/v) Sodium Azide
Alternative namessheep anti-Alanine Transaminase Antibody, biotin c
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NoteFor research use only
Application notesAnti-Alanine Transaminase has been assayed against 1.0 ug of Alanine Transaminase (ALT) in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Expiration Date12 months from date of receipt.
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