Alanine Transaminase antibody (Peroxidase)

• Catalog Number: orb345042
• Category: Antibodies
• Description: Alanine Transaminase antibody (Peroxidase)
• Species/Host: Sheep
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Porcine
• Isotype: IgG
• Immunogen: Alanine Transaminase (ALT) [Pig Heart]
• Concentration: 1 mg/mL
• Dilution range: ELISA: 1:10,000 - 1:40,000, WB: 1:1,000 - 1:5,000
• Form/Appearance: Lyophilized
• Purity: Alanine Transaminase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum as well as purified and partially purified Alanine Transaminase (ALT) [Pig Heart]. Cross reactivity against Alanine Transaminase (ALT) from other sources is unknown.
• Conjugation: HRP
• UniProt ID: P13191
• NCBI: 113565
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Alternative names: sheep anti-Alanine Transaminase HRP conjugated Ant
• Note: For research use only
• Application notes: Anti-Alanine Transaminase has been assayed against 1.0 µg of Alanine Transaminase (ALT) [Pig Heart] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:3,000 of the reconstitution concentration is suggested for this product.
• Expiration Date: 12 months from date of receipt.