Akt1 (phospho Tyr474) rabbit pAb

• Catalog Number: orb764361
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Akt1 (phospho-Tyr474)
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Monkey, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Immunogen: The antiserum was produced against synthesized peptide derived from human Akt around the phosphorylation site of Tyr474. AA range:431-480
• MW: 60
• Tested applications: ELISA, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-AKT1 antibody, anti-PKB antibody, anti-RAC an
• Note: For research use only

Western Blot analysis of 293T cells using Phospho-Akt1 (Y474) Polyclonal Antibody diluted at 1:1000.

Western Blot analysis of KB using Phospho-Akt1 (Y474) Polyclonal Antibody diluted at 1:1000.

Western Blot analysis of various cells using Phospho-Akt1 (Y474) Polyclonal Antibody diluted at 1:1000.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Akt (Phospho-Tyr474) Antibody.