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| Catalog Number | orb764129 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to Akt1 (phospho-Ser246) |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Concentration | 1 mg/ml |
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Akt around the phosphorylation site of Ser246. AA range:212-261 |
| MW | 56 |
| Tested applications | ELISA, IF, IHC-P, WB |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Anti-AKT1 antibody, anti-PKB antibody, anti-RAC an Read more... |
| Note | For research use only |
Western Blot analysis of various cells using Phospho-Akt1 (S246) Polyclonal Antibody diluted at 1:1000.
Western Blot analysis of HeLa cells using Phospho-Akt1 (S246) Polyclonal Antibody diluted at 1:1000.
Western Blot analysis of HEPG2 KB using Phospho-Akt1 (S246) Polyclonal Antibody diluted at 1:1000.
The picture was kindly provided by our customer, antibody was diluted at 1:500.
