AKT (phospho-S473) antibody

• Catalog Number: orb304681
• Category: Antibodies
• Description: Rabbit polyclonal antibody to AKT (phospho-S473)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IH, WB
• Reactivity: Bovine, Gallus, Human, Mouse, Porcine, Rat, Zebrafish
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S473 of human AKT protein. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1000, IHC-P: 1:100-200, IF/ICC: 1:100-500
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: AKT1; AKT2; AKT3
• Entrez: 207, 24185, 11651
• UniProt ID: P47196, P31750, P31749
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti PKB antibody, anti RAC antibody, anti RAC-alp
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Bourebaba, Lynda et al. The PTP1B inhibitor MSI-1436 ameliorates liver insulin sensitivity by modulating autophagy, ER stress and systemic inflammation in Equine metabolic syndrome affected horses Front Endocrinol (Lausanne), 14, 1149610 (2023)

PubMed: 37020593

Immunofluorescense analysis of MCF7 cells using AKT (phospho-S473) antibody

Western blot analysis of MCF7 PDGF-treated (Lane 1), NIH3T3 PDGF-treated (Lane 2) whole cell lysates using AKT (phospho-S473) antibody

Immunohistochemical analysis of formalin-fixed and paraffin-embedded human breast cancer tissue using AKT (phospho-S473) antibody