ADH1C Antibody

• Catalog Number: orb1264147
• Category: Antibodies
• Description: ADH1C Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This ADH1C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 231-260 amino acids from the Central region of human ADH1C.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50For IF starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 40 kDa
• Target: ADH1C
• UniProt ID: P00326
• NCBI: P00326
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Alcohol dehydrogenase 1C, Alcohol dehydrogenase su
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50For IF starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western blot analysis in T47D cell line lysates (35 ug/lane).

Western blot analysis of ADH1C using rabbit polyclonal ADH1C Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ADH1C gene.

ADH1C Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Confocal immunofluorescent analysis of ADH1C Antibody with T47D cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

Flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.