Acidic Cytokeratin Antibody (LMW / Type I)

• Catalog Number: orb2636715
• Category: Antibodies
• Description: The keratins are the typical intermediate filament proteins of epithelia, showing an outstanding degree of molecular diversity. Heteropolymeric filaments are formed by pairing of type I and type II molecules. In humans 54 functional keratin genes exist. They are expressed in highly specific patterns related to the epithelial type and stage of cellular differentiation. This antibody can detect high molecular weight CK10, CK14, CK15 and CK16 and low molecular weight CK19. Its usually used in a cytokeratin cocktail with type II cytokeratin antibody.
• Species/Host: Mouse
• Clonality: Recombinant
• Clone Number: rKRTL/6616
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: A synthetic peptide sequence common to Type I Cytokeratins was used as the immunogen for the recombinant Acidic Cytokeratin antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant Acidic Cytokeratin antibody is available for research use only.
• UniProt ID: Q7Z794
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant Acidic Cytokeratin antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

SDS-PAGE analysis of purified, BSA-free recombinant Acidic Cytokeratin antibody (clone rKRTL/6616) as confirmation of integrity and purity.

IHC staining of FFPE human sebaceous gland with recombinant Acidic Cytokeratin antibody (clone rKRTL/6616). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human skin tissue with recombinant Acidic Cytokeratin antibody (clone rKRTL/6616). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.