41708 antibody

• Catalog Number: orb20002
• Category: Antibodies
• Description: Goat polyclonal antibody to 41708
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Bovine, Human, Rat
• Dilution range: ELISA: 1:8000, WB: 0.3-1 μg/ml, IHC-P: 5 μg/ml
• Conjugation: Unconjugated
• MW: 90.5; 90.5
• Target: MARCH10
• Entrez: 162333
• Protein Sequence: SRNYPQPRTEE
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti MARCH10 antibody, anti membrane-associated ri
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx. 90 kDa band observed in Rat Testis lysates (calculated MW of 90.5 kDa according to Human NP_689811.2 and of 87.9 kDa according to Mouse NP_766156.2). Recommended concentration: 0.3-1 μg/ml. An additional band of unknown identity was also consistently observed at 37 kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Expiration Date: 12 months from date of receipt.

orb20002 (0.3ug/ml) staining of Rat Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemical staining of Human Testis using 41708 antibody