4-1BBL Antibody [AT113-2], Rabbit IgG

• Catalog Number: orb1671772
• Category: Antibodies
• Description: 4-1BBL Antibody [AT113-2], Rabbit IgG
• Clonality: Recombinant
• Clone Number: AT113-2
• Tested applications: Blocking, FC, WB
• Reactivity: Mouse
• Isotype: IgG kappa
• Immunogen: This antibody was raised by immunising LOU rats with soluble recombinant 4-1BBL:Fc fusion protein.
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: Tnfsf9
• UniProt ID: P41274
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CD137L, TNFSF9, 4-1BB ligand, 41BBL, CDw137L, homo
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of fixed RAW264.7 cells with anti-4-1BBL antibody AT113-2. Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix™ coverslips stained with the chimeric r version of AT113-2 (orb1671772) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671772- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.

Flow cytometry using the anti- 4-1BBL antibody AT113-2. RAW264.7 cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (3.0; isotype control - black line) or the r1 version of AT113-2 (orb1671772 - blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.