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Conjugations

 

Dyes

Abbreviation

Conjugation

Cy3

Indocarbocyanine 

Cy5

 

 

Indodicarbocyanine

 

Variant 1

Variant 2

Cy5.5

Cy7

Cyanine 5.5

Cyanine 7

FITC

Fluorescein Isothiocyanate

GFP

Green Fluorescent Protein

RBITC

Rhodamine B Isothiocyanate

TRITC

Thetramethylrhodamine Isothiocyanate

PE

Pycoerythrin

APC

Allophycocyanin

PerCP

Peridinin-Chlorophyll-Protein Complex

 

Other

Abbreviation

Conjugation

KLH

Keyhole Limpet hemocyanin

BSA

Bovine Serum Albumin

 

BTG

Bovine Thyroglobulin

 

HRP

Horseradish Peroxidase

 

AP

Alkaline Phosphatase

 

 

 

Not abbreviated

Gold

 

Biotin

 

Streptavidin

 

 

 

Descriptions

Dye

Absorbance/Excitation (nm)

Emission (nm)

Emission color

GFP

488

509

Green

FITC

494

520

Green

Cy3

550

570

Yellow

PE

496

578

Yellow

RBITC

540

625

Orange

TRITC

550

600

Orange

APC

650

660

Red

Cy5

650

670

Red

PerCP

482

678

Red

Cy5.5

675

694

Red

Cy7

743

767

Red

 

 

Dyes                           Dyes

 

Dyes                           Dyes

 

Dyes

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