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MMP9 antibody

Datasheet
Size Price Availability Qty
5 μg (Trial size)
$60
Available now for quick dispatch
20 μg (Trial size)
$128
Available now for quick dispatch
100 μg
$366
Usually dispatched within 5-10 working days
200 μg
$595
Usually dispatched within 5-10 working days
Shipping charges : Freight/Packing: $34

Rabbit polyclonal antibody to matrix metalloproteinases9 (MMP9) which belongs to the peptidase M10A family. It play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration and in bone osteoclastic resorption. Deficiency leads to Intervertebral disc disease (IDD).

Product Information

Review
PubMed 21454378
Category Primary antibodies
Product name MMP9 antibody
Catalog Number orb11064
Host Rabbit
Clonality Polyclonal
Tested applications IHC-P, IF/ICC, P-ELISA
Dilution range P-ELISA: 1:500-1000, IHC-P: 1:400-800, IF/ICC: 1:100-500
Concentration - 5 μg (in 100 μl): 0.05 mg/ml
- 20 μg (in 100 μl): 0.2 mg/ml
- 100 μg (in 200 μl): 0.5 mg/ml
- 200 μg (in 400 μl): 0.5 mg/ml
Reacts with Human, Mouse, Rat
UniProt ID P14780 (Human); P41245 (Mouse)
NCBI NP_004985.21; NM_004994.21
Entrez 4318
Conjugation Unconjugated
Immunogen KLH conjugated synthetic peptide derived between 621-697 amino acids of human MMP9
Purity Affinity purified by Protein A
MW 78 kDa
Isotype IgG
Form Liquid: 0.01M TBS (pH7.4), 0.5% BSA, 0.015% ProClin™ 300 and 25% glycerol
IHC notes Formalin-fixed paraffin-embedded tissue sections were subjected to antigen retrieval by treatment with 0.01 M Tris-HCl (pH 9.0), followed by incubation with primary MMP9 Antibody (Biorbyt; diluted 1:100)
Application notes Western Blot: Load 10 ug protein and block with 5% milk, MMP9 antibody is diluted 1:1000 This antibody can be used for WB, the correct bands. Predicted weight: 64/82/92kDa Tested weight: 64/82/92kDa
References Role of fibulin-5 in metastatic organ colonization.
Storage Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
Note For research use only.
Target MMP9
Alternative names
anti-92 KD antibody, anti-92 kDa type IV collagenase antibody, anti-92kDa gelatinase antibody, anti-92kDa type IV collagenase) antibody, anti-CLG4B antibody, anti-Collagenase Type 4 beta antibody, anti-Collagenase type IV antibody, anti-Collagenase type IV 92 KD antibody, anti-Collagenase type V antibody, anti-EC 3.4.24.35 antibody, anti-Gelatinase antibody, anti-Gelatinase 92 KD antibody, anti-Gelatinase beta antibody, anti-GelatinaseB antibody, anti-GELB antibody, anti-Matrix metallopeptidase 9 (gelatinase B antibody, anti-Matrix Metalloproteinase 9 antibody, anti-MMP-9 antibody, anti-MMP-9 antiobdy antibody, anti-Type V collagenase antibody, MMP9 Western Blot

MMP9 antibody Documents

MMP9 antibody Images Download
MMP9 antibody publication Download
Protocol for immunohistochemistry used to test MMP9 antibody Download
WB protocol to test the MMP9 Antibody Download
Western Blot on cells and tissues using MMP9 Antibody Download
Datasheet of MMP9 Antibody Download

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MMP9 antibody Customer Reviews

  1. Western Blot on mouse cells
    Posted on 30/06/2011

    The cells I used for the MMP9 Western blot were mouse osteosarcoma cells. I've been using the MMP9 antibody again recently and it's very clean. I load 10 ug protein and block with 5% milk.

  2. IF image of human endometrial epithelial cells grown in 3D
    Posted on 03/06/2011

    IF image of human endometrial epithelial cells grown in 3D; formalin fixed , paraffin embedded. 3 um sections were cut and following antigen retrieval, the section was stained with Rb anti-MMP9 (red) 1:100 overnight at 4oC. Nuclei stained with DAPI.

  3. MMP9 Western blot
    Posted on 07/10/2010

    I used the rabbit anti MMP9 antibody at 1:1000 on Western blot of mouse cell lysates and got one clear, specific band at around 90 kDa.


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Click image to enlarge
Immunohistochemical staining of paraffin embedded human stomach cancer tissue using MMP9 antibody (1:100, heat retrieval)
Immunofluorescence: IF image of human endometrial epithelial cells grown in 3D (1:100)
IHC-P of human stomach cancer tissue using MMP9 antibody (orb11064 at 1:200)
Western blot of mouse cell lysates using MMP9 antibody (1:1000, 5% milk, WB)
Paraffin-embedded rat ovary carcinoma tissue fixed with 4% paraformaldehyde. Antigen retrieval by boiling with citrate buffer. Blocking buffer is goat serum (37 degrees for 20 min.).MMP9 antibody at 1:200 dilution with overnight incubation period
 
 
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