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Human Leptin ELISA kit

480 Assays
Usually dispatched within 5-10 working days
192 Assays
Usually dispatched within 5-10 working days
96 Assays
Usually dispatched within 5-10 working days.
Shipping charges : Freight/Packing: $51

Human Leptin ELISA Kit for Serum, Plasma, Cell Culture Supernatant and Urine.

Product Information

Category ELISA kits
Product name Human Leptin ELISA kit
Catalog Number orb78861
Tested applications ELISA
Reacts with Human
Dynamic range 2 pg/ml - 400 pg/ml
Specificity Human
Sensitivity 2 pg/ml
Kits Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Assay Diluent(s)
  • Biotinylated Detection Antibody
  • HRP-Streptavidin
  • TMB One-Step Substrate
  • Stop Solution
  • Lyophilized Standard
Storage Short term store at 4°C. for long term storage aliquot and store at 20°C. or 80°C.Avoid repeated freeze/thaw cycles
Note For research use only.
Protocol details
  • » Prepare all reagents, samples and standards as instructed in the manual.
  • » Add 100 μl of standard or sample to each well.
  • » Incubate 2.5 h at RT or O/N at 4°C.
  • » Add 100 μl of prepared biotin antibody to each well.
  • » Incubate 1 h at RT.
  • » Add 100 μl prepared streptavidin solution to each well.
  • » Incubate 45 min at RT.
  • » Add 100 μl TMB One-Step Substrate Reagent to each well.
  • » Incubate 30 min at RT.
  • » Add 50 μl Stop Solution to each well.
  • » Read plate at 450 nm immediately.
Target Leptin ELISA kit
Alternative names
Human Leptin ELISA kit

Human Leptin ELISA kit Documents

orb78861 Product ManualDownload

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