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Human Insulin ELISA kit

192 Assays
Usually dispatched within 5-10 working days
96 Assays
Usually dispatched within 5-10 working days.
480 Assays
Usually dispatched within 5-10 working days
Shipping charges : Freight/Packing: $51

Human Insulin ELISA Kit for Serum, Plasma, Cell Culture Supernatants and Urine.

Product Information

Category ELISA kits
Product name Human Insulin ELISA kit
Catalog Number orb78858
Tested applications ELISA
Reacts with Human
Dynamic range 4 µIU/ml - 300 pIU/ml
Specificity Human
Sensitivity 4 μIU/ml
Kits Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Assay Diluent(s)
  • Biotinylated Detection Antibody
  • HRP-Streptavidin
  • TMB One-Step Substrate
  • Stop Solution
  • Lyophilized Standard
Storage Store at 4°C. For long term storage aliquot and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles.
Note For research use only.
Protocol details
  • » Prepare all reagents, samples and standards as instructed in the manual.
  • » Add 100 μl of standard or sample to each well.
  • » Incubate 2.5 h at RT or O/N at 4°C.
  • » Add 100 μl of prepared biotin antibody to each well.
  • » Incubate 1 h at RT.
  • » Add 100 μl prepared streptavidin solution to each well.
  • » Incubate 45 min at RT.
  • » Add 100 μl TMB One-Step Substrate Reagent to each well.
  • » Incubate 30 min at RT.
  • » Add 50 μl Stop Solution to each well.
  • » Read plate at 450 nm immediately.
Target Insulin ELISA kit
Alternative names
Human Insulin ELISA kit

Human Insulin ELISA kit Documents

orb78858 Product ManualDownload

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