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Human CXCL16 ELISA Kit

Datasheet
SizePriceAvailabilityQty
96 well
$576
Usually dispatched within 5-10 working days.
Shipping charges : Freight/Packing: $51

Sandwich Elisa kit for Quantitative Detection for Human CXCL16, Chemokine C-X-C motif ligand 16 CXCL16 is a small cytokine belonging to the CXC chemokine family. Larger than other chemokines with 254 amino acids, CXCL16 is composed of a CXC chemokine domain, a mucin-like stalk, a transmembrane domain and a cytoplasmic tail containing a potential tyrosine phosphorylation site that may bind SH2. These are unusual features for a chemokine, and allow CXCL16 to be expressed as a cell surface bound molecule, as well as a soluble chemokine. CXCL16 is produced by dendritic cells found in the T cell zones of lymphoid organs, and by cells found in the red pulp of the spleen. Cells that bind and migrate in response to CXCL16 include several subsets of T cells, and natural killer T NKT cells. CXCL16 interacts with the chemokine receptorCXCR6, also known as Bonzo. The novel CXC-chemokine ligand 16 CXCL16 functions as transmembrane adhesion molecule on the surface of APCs and as a soluble chemoattractant for activated T cells. Expression of CXCL16 is induced by the inflammatory cytokines IFN-gamma and TNF-alpha. The gene for human CXCL16 is located on chromosome 17.

Product Information

Category ELISA kits
Product name Human CXCL16 ELISA Kit
Catalog Number orb96783
Tested applications ELISA
Reacts with Human
Dynamic range 93.7pg/ml-6000pg/ml
Specificity Natural and recombinant human CXCL16
Sensitivity < 10pg/ml
Experimental notes There is no detectable cross-reactivity with other relevant proteins.
Storage Store at 4°C.for 6 months, at -20°C. for 12 months. Avoid multiple freeze-thaw cycles
Note For research use only.
Target CXCL16
Application notes The human CXCL16 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Human CXCL16 specific-specific polyclonal antibodies were precoated onto 96-well plates. The human specific detection monoclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CXCL16 amount of sample captured in plate.
Alternative names
No

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The standard curve illustrates about the dynamic range of Human CXCL16 ELISA Kit using various concentrations.
 
 
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