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CD133 antibody

Datasheet
SizePriceAvailabilityQty
100 μg
$366
Usually dispatched within 3-7 working days.
200 μg
$595
Usually dispatched within 3-7 working days.
Shipping charges : Freight/Packing: $34

Rabbit polyclonal antibody to CD133, which is also known as prominin or AC133. CD133 is a marker frequently found on multipotent progenitor cells, including immature hematopoietic stem and progenitor cells. The protein has been extensively used as a stem cell marker for normal and cancerous tissues.

Product Information

Review
PubMed 25421411; 26028817
Category Primary antibodies
Product name CD133 antibody
Catalog Number orb99113
Host Rabbit
Clonality Polyclonal
Tested applications IF/ICC, IHC-P, P-ELISA, WB
Dilution range IHC-P:1:200, WB: 1:500, IF/ICC: 1:200
Concentration - 100 μg (in 100 μl): 1 mg/ml
- 200 μg (in 200 μl): 1 mg/ml
Reacts with Human, Mouse, Rat
UniProt ID O43490 (Human); O54990 (Mouse)
NCBI NP_001139319.11; NM_001145847.11
Entrez 8842
Conjugation Unconjugated
Immunogen KLH conjugated synthetic peptide derived from human CD133. Please contact us for the exact immunogen sequence. The peptide is available as orb374954.
Purity Polyclonal antibodies are purified by peptide affinity chromatography
MW 95 kDa
Isotype IgG
Form 10 mM PBS, 0.02% sodium azide, 10 mg/ml BSA
Specificity Human;Mouse;Rat. No cross reactivity with other proteins.
Experimental notes Add 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Application notes Several IHC experiments have been done on human cancer tissue. Please review the information on the product page under "Additional Information"
References 1. Endothelial progenitor cells proliferated via MEK-dependent p42 MAPK signaling pathway.
2. Expression and activation of EGFR and STAT3 during the multistage carcinogenesis of intrahepatic cholangiocarcinoma induced by 3'-methyl-4 dimethylaminoazobenzene in rats.
3. Isolation and phenotypic characterization of cancer stem-like side population cells in colon cancer.
4. Influence of interferon-a on the expression of the cancer stem cell markers in pancreatic carcinoma cells.
5. CD133 expression in circulating tumor cells from breast cancer patients: Potential role in resistance to chemotherapy
6. Impact of CD133 positive stem cell proportion on survival in patients with glioblastoma multiforme.
7. An innovative three-dimensional gelatin foam culture system for improved study of glioblastoma stem cell behavior.
8. Correlation of altered expression of the autophagy marker LC3B with poor prognosis in astrocytoma.
9. Nrf2 Is Involved in Maintaining Hepatocyte Identity during Liver Regeneration.
10.Descriptive analysis of tumor cells with stem like phenotypes in metastatic and benign adrenal tumors.
11. Restoration of erectile function with intracavernous injections of endothelial progenitor cells after bilateral cavernous nerve injury in rats.
12. Nanovehicles as a novel target strategy for hyperthermic intraperitoneal chemotherapy: a multidisciplinary study of peritoneal carcinomatosis.
13. Circulating Tumor Cell Composition in Renal Cell Carcinoma.
14.CXCR4 is involved in CD133-induced EMT in non-small cell lung cancer.
Storage After reconstitution store at 4°C.for up to two weeks. For long term storage, aliquot and store at -20°C. Avoid freeze/thaw cycles.
Note For research use only.
Target CD133
Alternative names
AC133 antibody,gen AC133 antibody, CD133 antibody, CD133 Partial Recombinant Protein antibody, CORD12 antibody, Hematopoietic stem cellgen antibody, hProminin antibody, MCDR2 antibody, MSTP061 antibody, PROM antibody, PROM1 antibody, Prominin I antibody, Prominin like protein 1 precursor antibody, Prominin mouse like 1 antibody, prominin1 antibody, PROML1 antibody, RP41 antibody, STGD4 antibody, AC133 antibody, Antigen AC133 antibody, Hematopoietic stem cell antigen antibody, hProminin antibody, PROM1 antibody, Prominin I antibody, Prominin like protein 1 precursor antibody, Prominin mouse like 1 antibody, prominin1 antibody, PROML1 antibody, CD133 antibody, CORD12 antibody, MCDR2 antibody, MSTP061 antibody, PROML1 antibody, RP41 antibody, STGD4.

CD133 antibody Documents

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CD133 antibody PublicationsDownload
CD133 PublicationDownload
CD133_IHC_ReportDownload

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CD133 antibody Customer Reviews

  1. CD133 antibody used IHC-P
    Posted on 23/01/2017

    Immunohistochemical staining of humna lung cancer using CD133 antibody.

  2. Western Blot using CD133 for human cells
    Posted on 12/01/2017

    Please find attached the WB for CD133.
    Its done in human cells- but it also works in mouse.
    I used 5% BSA /TNT for blocking. Antibody was used 1:500 over night in TNT.
    Secondary Amersham 1:10000 in BSA /TNT 1h.
    Developing time approx. 30sec- 1min.
    Amount of lysate: 25µg/ lane.

  3. CD133 antibody (18124) on feline FFPE tissues
    Posted on 12/01/2017

    3 different antigen retrievals (citrate, EDTA, and protease) were used investigating CD133 antibody. universal non-specific staining block and quenching of endogenous peroxydase were performed. 1:200 dilution overnight at 4C. Universal anti-rabbit polymer secondary ab was used. DAB for chemical reaction.

    Unfortunately, literarly no staining was observed on feline kidney proximal tubules and tumor. Weak but distinduishable staining was seen on mouse retina. Can't complain about the result as the company doesn't say this ab works on feline.

  4. CD133 antibody review, various antigen retrieval
    Posted on 12/01/2017

    During testing the antibodies and after pre-treatment in MW (citrate buffer, pH6) or in High pH (Dako) we observed non-specific binding of orb10288 and orb43280 in cytoplasm and also in nuclei. Therefore we choosed the orb18124 CD133 antibody. The MW pre-treatment caused also non-specific staining of nuclei but the High pH was OK. The best results we obtained were without any pre-treatment, dilution 1:200. There the cytoplasic membrane positivity was detected in kidney and lymph nodes tissues for CD133 antibody

  5. Western blot analysis of Human cancer cell lysates using CD133 (orb18124) antibody
    Posted on 12/01/2017

    Customer review:
    Western blot analysis of Human cancer cell lysates using CD133 (orb18124) antibody

    Experiment details:
    Protein Lysate: Human tongue cancer cell line.
    Blocking: 5% skimmed milk in TBS buffer for 2 Hours.
    Primary Ab: 1:1000 in 2.5% Skimmed milk in 1X TBST for Overnight at 4 degree Celsius.
    Secondary Ab: Anti Rabbit,1:3000 in 2.5% Milk for 1 Hour.

    Result:
    In lane 1 and 2 Glucosylated form of CD133 increasing (band above 130kDa), while non glycosylated form is decreasing (Band lower to 100kDa) on trearment to AW13516 cell line. In lane 3 and 4 No change in glycosylated and non glycosylated form of CD133 after treatment.

    Conclusion:
    CD133 antibody from Biorbyt company (orb18124) is able to label both the glycosylated and unglycosylated form of the CD133 protein in our Human cancer cell lysates.

  6. Western Blot using CD133 for human cells
    Posted on 12/01/2017

    Please find attached the WB for CD133.
    Its done in human cells- but it also works in mouse.
    I used 5% BSA /TNT for blocking. Antibody was used 1:500 over night in TNT.
    Secondary Amersham 1:10000 in BSA /TNT 1h.
    Developing time approx. 30sec- 1min.
    Amount of lysate: 25ug/ lane.


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Click image to enlarge
IF analysis of mouse skin tissue using CD133 antibody (Dilution of primary antibody 1:200)
Western blot analysis of Mouse large intestine (Lane 1), Mouse spinal cord (Lane 2) using CD133 antibody (Dilution of primary antibody 1:200)
Immunohistochemical staining of human mammary fibroma tissue using anti-CD206 (dilution of primary antibody - 1:100)
IHC-P analysis of human colonic adenocarcinoma tissue using CD133 antibody (Primary antibody at 1:100)
IHC-P analysis of human muscle tissue using CD133 antibody (Primary antibody diluted to 1:100)
Immunofluorescent analysis of mouse brain tissue using CD133 antibody (Primary antibody diluted to 1:200)
 
 
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