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Caspase 3 antibody

Datasheet
Size Price Availability Qty
5 μg (Trial size)
$60
Available now for quick dispatch
20 μg (Trial size)
$128
Available now for quick dispatch
100 μg
$366
Usually dispatched within 5-10 working days
200 μg
$595
Usually dispatched within 5-10 working days
Shipping charges : Freight/Packing: $34

Rabbit polyclonal antibody to caspase 3 is also known as apopain and whose expression can be detected in the tonsils and skeletal muscles but it is highly expressive in the spleen and kidney. It is found in the death-inducing signaling complex in plasma membrane. This protein is extensively involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis, it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a 216-Asp-|-Gly-217 bond. Caspase 3 cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain.

Product Information

Review
PubMed 23203036; 25124665
Category Primary antibodies
Product name Caspase 3 antibody
Catalog Number orb10237
Host Rabbit
Clonality Polyclonal
Tested applications WB, IHC-P, IF/ICC, FACS, P-ELISA
Dilution range WB: 1:500-2000, P-ELISA: 1:500-1000, IHC-P: 1:400-800, FACS: 2μg/Test, IF/ICC: 1:100-500
Concentration - 5 μg (in 100 μl): 0.05 mg/ml
- 20 μg (in 100 μl): 0.2 mg/ml
- 100 μg (in 200 μl): 0.5 mg/ml
- 200 μg (in 400 μl): 0.5 mg/ml
Reacts with Human, Mouse, Rat
Predicted reactivity Horse, Sheep, Cow, Pig, Dog
UniProt ID P42574 (Human); P70677 (Mouse); P55213 (Rat)
NCBI NP_004337.21; NM_004346.31
Entrez 836
Conjugation Unconjugated
Immunogen KLH conjugated synthetic peptide derived between 33178 amino acids of human Caspase 3
Purity Affinity purified by Protein A
MW 28 kDa
Isotype IgG
Form Liquid: 0.01M TBS (pH7.4), 0.5% BSA, 0.015% ProClin™ 300 and 25% glycerol
References 1. Phorbol esters isolated from jatropha meal induced apoptosis-mediated inhibition in proliferation of chang and vero cell lines.
2. Nascent histamine induces α-synuclein and caspase-3 on human cells.
3. Combination of gemcitabine and erlotinib inhibits recurrent pancreatic cancer growth in mice via the JAK-STAT pathway
4.Emodin protects hyperglycemia-induced injury in PC-12 cells by up-regulation of miR-9.
Storage Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
Note For research use only.
Target Caspase 3
Alternative names
anti-A830040C14Rik antibody, anti-apop antibody, anti-Apopain antibody, anti-Apopain precursor antibody, anti-CASP3 antibody, anti-Casp3a antibody, anti-Caspase 3 apoptosis related cysteine protease antibody, anti-Caspase 3 p12 subunit antibody, anti-Caspase 3 p17 subunit antibody, anti-Caspase3 antibody, anti-CPP32 antibody, anti-CPP32B antibody, anti-Cysteine protease CPP32 antibody, anti-Human cysteine protease CPP32 isoform alpha mRNA complete cds antibody, anti-ICE3 antibody, anti-LICE antibody, anti-PARP cleavage protease antibody, anti-SCA-1 antibody, anti-SREBP cleavage activity 1 antibody, anti-Yama protein antibody

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Caspase 3 antibody Customer Reviews

  1. Use of antibody in western blot
    Posted on 28/03/2018

    Caspase 3 antibody was used for the western blot analysis.

  2. immunohistochemical staining using primary antibody
    Posted on 26/02/2018

    Paraffin embedded tissue was deparaffinized and incubated with primary antibody at 1:200 dilution followed ny secondary antibody for Immunohistochemical staining.

  3. Caspase 3 antibody used in Immunofluorescense
    Posted on 07/04/2015

    Good results in Immunofluorescense analysis of human glioma and osteosarcoma cells using Caspase 3 antibody (antibody dilution at 1:100)

  4. Western blotting was employed to examine the expression of proteins related to pStat3 signaling pathway
    Posted on 07/10/2014

    Total cell lysates were prepared with tween-20 lysis buffer and sonication. then proteins were seperated by SDS-PAGE and transferred to polyvinylidene difluoride membrane, The membrane were washed, blocked and incubated with the specific priamry antibody caspase-3 antibody used with 1:1000 concentration

  5. Western blotting analysis showed that EMMPRIN knockdown was associated with downregulation of cell cycle and increase in cleavage of caspase-3 and PARP.
    Posted on 07/10/2014

    For Western blot analysis:

    Celles were harvested form flasks, and lysed with ice-colod lysis buffer (tris HCL), pH 7,4 , 150 mN NaCl, 1mM MgCl, 100ug/ml PMSF, nad1& Triton X-100) For 30 min on ice.

    Cell lysate was then collected after centrifugation at 4'C, 12,000 rpm for 5 min.

    50 ug of lysate proteins were seperated on 10& SDS-PAGE gels, and transferrred on to PVDF membranes.

    Caspase 3 (concentration: 1:1000) used as Primary antibody.

    Conclusion:

    Western blotting analysis showed that EMMPRIN knockdown was associated with downregulation of cell cycle- and apoptosis-related molecules including cyclin D1, cyclin E, as well as increase in cleavage of caspase-3 and PARP.


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Click image to enlarge
Western blot analyses for PKC-δ, Caspase-3 and GAPDH protein expression in MCF-7 cell line
Immunohistochemical analysis of paraffin-embedded rabbit heart tissue using Caspase 3 antibody
Paraffin-embedded rat brain tissue fixed with 4% paraformaldehyde. Antigen retrieval by boiling with citrate buffer. Blocking buffer is goat serum (37 degrees for 20 min.). Anti Caspase 3 at 1:200 dilution with a overnight incubation period
Western blot analysis of rat brain lysates using Caspase 3 antibody
 
 
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